Nick Kroll

Nick Birch / Maury the Hormone Monster / Coach Steve / Lola Skumpy / Rick the Hormone Monster (voice)

John Mulaney

Andrew Glouberman (voice)

Jessi Klein

Jessi Glaser (voice)

Jason Mantzoukas

Jay Bilzerian (voice)

Maya Rudolph

Diane Birch / Connie the Hormone Monstress (voice)

Fred Armisen

Elliot Birch (voice)

Jordan Peele

Ghost of Duke Ellington (voice)

Andrew Rannells

Matthew (voice)